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Genomic and metabolomic analysis of with enhanced poly--glutamic acid production through atmospheric and room temperature plasma mutagenesis

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 12,   Pages 1751-1760 doi: 10.1007/s11705-022-2211-x

Abstract: Atmospheric and room temperature plasma was applied in this study to conduct mutation breeding of Bacilluslicheniformis CGMCC 2876, and a mutant strain M32 with an 11% increase in poly-γ-glutamic

Keywords： ARTP mutagenesis     Bacillus licheniformis     poly-γ-glutamic acid     metabolomics    

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A meta-analysis of randomized trials of maintenance bacillus Calmette-Guerin instillation efficacy against

PAN Jiangang, ZHOU Xing, CHEN Zhiguang, HAN Ruifa

Frontiers of Medicine 2008, Volume 2, Issue 3,   Pages 259-263 doi: 10.1007/s11684-008-0049-3

Abstract: Meta-analysis was used to determine whether maintenance intravesical bacillus Calmette-Guerin (BCG) could
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Can intravesical bacillus Calmette-Guérin reduce recurrence in patients with non-muscle invasive bladder

Jiangang Pan,Mo Liu,Xing Zhou

Frontiers of Medicine 2014, Volume 8, Issue 2,   Pages 241-249 doi: 10.1007/s11684-014-0328-0

Abstract: Bacillus Calmette-Guérin (BCG) instillation and maintenance is considered as the standard adjuvant

Keywords： non-muscle invasive bladder cancer     bacillus Calmette-Guérin (BCG)     meta-analysis    

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Characterization of Bacillus amyloliquefacien contaminating 75% alcohol disinfectant

ZHANG Wanming, YUAN Yuesha, BIAN Cangli, TU Xianyu, ZHANG Wen, HUANG Huqiang, WANG Lan

Frontiers of Medicine 2008, Volume 2, Issue 1,   Pages 113-116 doi: 10.1007/s11684-008-0021-2

Abstract: The clinical characterization of Bacillus amyloliquefacien contaminating 75% alcohol disinfectants wereTheir total number was more than 800 cfu/mL and they were identified as Bacillus amyloliquefacien.It was suggested that alcohol disinfectant fluid was easily contaminated by Bacillus amyloliquefacien
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Testing of tuberculosis infection among Chinese adolescents born after terminating the Bacillus Calmette

Hengjing Li, Henan Xin, Shukun Qian, Xiangwei Li, Haoran Zhang, Mufei Li, Boxuan Feng, Qi Jin, Lei Gao

Frontiers of Medicine 2017, Volume 11, Issue 4,   Pages 528-535 doi: 10.1007/s11684-017-0573-0

Abstract:

The prevalence of tuberculosis infection among adolescents born after terminating the Bacillus Calmette

Keywords： tuberculin skin test     interferon-γ release assays     adolescent     agreement    

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Use of dry yeast cells as a cheap nitrogen source for lactic acid production by thermophilic

Kim Yng Ooi, Jin Chuan Wu

Frontiers of Chemical Science and Engineering 2015, Volume 9, Issue 3,   Pages 381-385 doi: 10.1007/s11705-015-1534-2

Abstract: Dry yeast cells (DYC) were used as a cheap nitrogen source to replace expensive yeast extract (YE) for L-lactic acid production by thermophilic . Cassava starch (200 g·L ) was converted to L-lactic acid by simultaneous saccharification and fermentation using WCP10-4 at 50 °C in the presence of 20 g·L of DYC, giving 148.1 g·L of L-lactic acid at 27 h with a productivity of 5.5 g·L ·h and a yield of 92%. In contrast, 154.4 g·L of lactic acid was produced at 24 h with a productivity of 6.4 g·L ·h and a yield of 96% when equal amount of YE was used under the same conditions. Use of pre-autolyzed DYC at 50 °C for overnight slightly improved the lactic acid titer (154.5 g·L ) and productivity (7.7 g·L ·h ) but gave the same yield (96%).

Keywords： L-lactic acid     thermophilic strain     Bacillus coagulans     dry yeast cells     autolysis     fermentation    

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Chromium phytoextraction and physiological responses of the hyperaccumulator Swartz to plant growth-promoting rhizobacterium inoculation

Frontiers of Environmental Science & Engineering 2023, Volume 17, Issue 1, doi: 10.1007/s11783-023-1609-0

Abstract:

● Improved Cr phytoextration efficiency was achieved by B. cereus inoculation.

Keywords： Hexavalent chromium     Hyperaccumulator     Rhizobacteria     Leersia hexandra Swartz     Bacillus cereus    

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Physicochemical Properties and Bioactivities of Rice Beans Fermented by Bacillus amyloliquefaciens Article

Shan Wu,  Shuai Lu, Jun Liu, Shaoqing Yang, Qiaojuan Yan, Zhengqiang Jiang

Engineering 2021, Volume 7, Issue 2,   Pages 219-225 doi: 10.1016/j.eng.2020.10.010

Abstract: physicochemical properties and bioactivities of rice beans (Vigna umbellata) fermented by BacillusRice beans fermented by Bacillus amyloliquefaciens may serve as a functional food with potential

Keywords： Solid-state fermentation     Rice beans     Bacillus amyloliquefaciens     Antithrombotic activity     Antioxidant    

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Roles of acid-producing bacteria in anaerobic digestion of waste activated sludge

Sijia Ai, Hongyu Liu, Mengjie Wu, Guangming Zeng, Chunping Yang

Frontiers of Environmental Science & Engineering 2018, Volume 12, Issue 6, doi: 10.1007/s11783-018-1050-y

Abstract:

Bacillus coagulans improved TCOD removal

The optimal inoculum concentration of Bacillus

Keywords： Anaerobic digestion     Waste activated sludge     Acid-producing bacteria     Bacillus coagulans    

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Effect of calcium lactate on compressive strength and self-healing of cracks in microbial concrete

Kunamineni VIJAY, Meena MURMU

Frontiers of Structural and Civil Engineering 2019, Volume 13, Issue 3,   Pages 515-525 doi: 10.1007/s11709-018-0494-2

Abstract: This paper presents the effect on compressive strength and self-healing capability of bacterial concrete with the addition of calcium lactate. Compared to normal concrete, bacterial concrete possesses higher durability and engineering concrete properties. The production of calcium carbonate in bacterial concrete is limited to the calcium content in cement. Hence calcium lactate is externally added to be an additional source of calcium in the concrete. The influence of this addition on compressive strength, self-healing capability of cracks is highlighted in this study. The bacterium used in the study is and was added to both spore powder form and culture form to the concrete. spore powder of 2 million cfu/g concentration with 0.5% cement was mixed to concrete. Calcium lactates with concentrations of 0.5%, 1.0%, 1.5%, 2.0%, and 2.5% of cement, was added to the concrete mixes to test the effect on properties of concrete. In other samples, cultured with a concentration of 1×10 cells/mL was mixed with concrete, to study the effect of bacteria in the cultured form on the properties of concrete. Cubes of 100 mm×100 mm×100 mm were used for the study. These cubes were tested after a curing period of 7, 14 and 28 d. A maximum of 12% increase in compressive strength was observed with the addition of 0.5% of calcium lactate in concrete. Scanning electron microscope and energy dispersive X-ray spectroscopy examination showed the formation of ettringite in pores; calcium silicate hydrates and calcite which made the concrete denser. A statistical technique was applied to analyze the experimental data of the compressive strengths of cementations materials. Response surface methodology was adopted for optimizing the experimental data. The regression equation was yielded by the application of response surface methodology relating response variables to input parameters. This method aids in predicting the experimental results accurately with an acceptable range of error. Findings of this investigation indicated the influence of added calcium lactate in bio-concrete which is quite impressive for improving the compressive strength and self-healing properties of concrete.

Keywords： calcium lactate     bacillus subtilis     compressive strength     self-healing of cracks    

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The biosorption of Cr(VI) ions by dried biomass obtained from a chromium-resistant bacterium

Paul Fabrice NGUEMA,Zejiao LUO,Jingjing LIAN

Frontiers of Chemical Science and Engineering 2014, Volume 8, Issue 4,   Pages 454-464 doi: 10.1007/s11705-014-1456-4

Abstract: The biosorption potential of many different kinds of biomaterials has been widely studied. However, there is little data on the biosorption mechanism of Cr(VI) by dried biomass. So the bio-removal of Cr(VI) ions from aqueous solutions was investigated using dried biomass from a chromium-resistant bacterium. The bacterium was isolated from dewatered sludge samples that were obtained from a sewage treatment plant. Equilibrium and kinetic experiments were performed at different metal concentrations, pH values, and biosorbents dosages. The biomass was characterized using scanning electron microscopy and energy-dispersive X-ray spectroscopy. The functional groups in the biomass which may play a role in the biosorption process were identified by Fourier transform infrared spectroscopy. The biosorption process was found to be highly pH dependent and the optimum pH for the adsorption of Cr(VI) was 2.0±0.3 at 30±2 °C. The experimental data fit well with Langmuir and Freundlich models as well as a pseudo-second order kinetic model. The mechanism for the biosorption was also studied by fitting the kinetic data with an intra-particle diffusion model and a Boyd plot. External mass transfer was found to be the rate-determining step for the adsorption process. Biosorption could be an alternative mechanism besides bio-oxidation and bio-reduction for the bioremediation of heavy metals.

Keywords： Cr(VI)     biomass     Bacillus cereus Pf-1     biosorption capacity     biosorbents    

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Effect of pH on biologic degradation of

Hongjing LI, Mengli HAO, Jingxian LIU, Chen CHEN, Zhengqiu FAN, Xiangrong WANG

Frontiers of Environmental Science & Engineering 2012, Volume 6, Issue 2,   Pages 224-230 doi: 10.1007/s11783-011-0314-6

Abstract: In this paper, the effect of pH on biological degradation of by alga-lysing bacteria in laboratory-scale sequencing batch biofilm reactors (SBBRs) was investigated. After 10 d filming with waste activated sludge, the biological film could be formed, and the bioreactors in which laid polyolefin resin filler were used to treat algal culture. By comparing the removal efficiency of chlorophyll at different aerobic time, the optimum time was determined as 5 h. Under pH 6.5, 7.5, and 8.5 conditions, the removal rates of were respectively 75.9%, 83.6%, and 78.3% (in term of chlorophyll ), and that of Chemical Oxygen Demand (COD ) were 30.6%, 35.8%, and 33.5%. While the removal efficiencies of ammonia nitrogen ( ) were all 100%. It was observed that the sequence of the removal efficiencies of algae, and organic matter were pH 7.5>pH 8.5>pH 6.5. The results showed that the dominant alga-lysing bacteria in the SBBRs was strain HM-01, which was identified as sp. by Polymerase Chain Reaction (PCR) amplification of the 16S rRNA gene, Basic Local Alignment Search Tool (BLAST) analysis, and comparison with sequences in the GenBank nucleotide database. The algicidal activated substance which HM-01 strain excreted could withstand high temperature and pressure, also had better hydrophily and stronger polarity.

Keywords： biological degradation     alga-lysing bacteria     sequencing batch biofilm reactor (SBBR)     16S rRNA     Bacillus    

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Combination of ARTP mutagenesis and color-mediated high-throughput screening to enhance 1-naphthol yield from microbial oxidation of naphthalene in aqueous system

Chenggang Qiu, Alei Zhang, Sha Tao, Kang Li, Kequan Chen, Pingkai Ouyang

Frontiers of Chemical Science and Engineering 2020, Volume 14, Issue 5,   Pages 793-801 doi: 10.1007/s11705-019-1876-2

Abstract: Strain QCG of the aerobic bacteria is capable of producing 1-naphthol from naphthalene, this strain was first isolated and characterized in this study. Strain QCG was mutagenized to enhance 1-naphthol production, using atmospheric and room temperature plasma (ARTP) technology. Then, a microbial clone screening system was used to accelerate the operation. Meanwhile, a novel color-mediated high-throughput screening using 4-aminoantipyrine was performed to screen mutants. The optimal mutant strain QCG4 produced 19.58±0.34 mg∙L 1-naphthol from naphthalene that was 47.32% higher than that of the original strain (13.29±0.28 mg∙L ). In addition, the optimal conditions for 1-naphthol production via whole-cell catalysis of strain QCG4 were determined to be an OD of 40, 150 mg∙L naphthalene, and 7.5% dimethyl formamide as a co-solvent at pH 7.5 and 26°C for 3 h, resulting in 41.18±0.12 mg∙L 1-naphthol, i.e., the mutant strain produces a 2.1-fold higher yield compared to the original strain.

Keywords： Bacillus cereus QCG     naphthalene     1-naphthol     ARTP mutagenesis     high-throughput screening     4-aminoantipyrine    

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Remodeling Isoprene Pyrophosphate Metabolism for Promoting Terpenoids Bioproduction Article

Xianhao Xu, Xueqin Lv, Shixiu Cui, Yanfeng Liu, Hongzhi Xia, Jianghua Li, Guocheng Du, Zhaofeng Li, Rodrigo Ledesma-Amaro, Jian Chen, Long Liu

Engineering 2023, Volume 28, Issue 9,   Pages 166-178 doi: 10.1016/j.eng.2023.03.019

Abstract: flux distribution between the central metabolism and the IPP supply hinders IPP accumulation in Bacillus

Keywords： Terpenoids     Genetic circuits     Bacillus subtilis     Isoprene pyrophosphate    

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Title Author Date Type Operation

Genomic and metabolomic analysis of with enhanced poly--glutamic acid production through atmospheric and room temperature plasma mutagenesis

Journal Article

A meta-analysis of randomized trials of maintenance bacillus Calmette-Guerin instillation efficacy against

PAN Jiangang, ZHOU Xing, CHEN Zhiguang, HAN Ruifa

Journal Article

Can intravesical bacillus Calmette-Guérin reduce recurrence in patients with non-muscle invasive bladder

Jiangang Pan,Mo Liu,Xing Zhou

Journal Article

Characterization of Bacillus amyloliquefacien contaminating 75% alcohol disinfectant

ZHANG Wanming, YUAN Yuesha, BIAN Cangli, TU Xianyu, ZHANG Wen, HUANG Huqiang, WANG Lan

Journal Article

Testing of tuberculosis infection among Chinese adolescents born after terminating the Bacillus Calmette

Hengjing Li, Henan Xin, Shukun Qian, Xiangwei Li, Haoran Zhang, Mufei Li, Boxuan Feng, Qi Jin, Lei Gao

Journal Article

Use of dry yeast cells as a cheap nitrogen source for lactic acid production by thermophilic

Kim Yng Ooi, Jin Chuan Wu

Journal Article

Chromium phytoextraction and physiological responses of the hyperaccumulator Swartz to plant growth-promoting rhizobacterium inoculation

Journal Article

Physicochemical Properties and Bioactivities of Rice Beans Fermented by Bacillus amyloliquefaciens

Shan Wu,  Shuai Lu, Jun Liu, Shaoqing Yang, Qiaojuan Yan, Zhengqiang Jiang

Journal Article

Roles of acid-producing bacteria in anaerobic digestion of waste activated sludge

Sijia Ai, Hongyu Liu, Mengjie Wu, Guangming Zeng, Chunping Yang

Journal Article

Effect of calcium lactate on compressive strength and self-healing of cracks in microbial concrete

Kunamineni VIJAY, Meena MURMU

Journal Article

The biosorption of Cr(VI) ions by dried biomass obtained from a chromium-resistant bacterium

Paul Fabrice NGUEMA,Zejiao LUO,Jingjing LIAN

Journal Article

Effect of pH on biologic degradation of

Hongjing LI, Mengli HAO, Jingxian LIU, Chen CHEN, Zhengqiu FAN, Xiangrong WANG

Journal Article

Combination of ARTP mutagenesis and color-mediated high-throughput screening to enhance 1-naphthol yield from microbial oxidation of naphthalene in aqueous system

Chenggang Qiu, Alei Zhang, Sha Tao, Kang Li, Kequan Chen, Pingkai Ouyang

Journal Article

Remodeling Isoprene Pyrophosphate Metabolism for Promoting Terpenoids Bioproduction

Xianhao Xu, Xueqin Lv, Shixiu Cui, Yanfeng Liu, Hongzhi Xia, Jianghua Li, Guocheng Du, Zhaofeng Li, Rodrigo Ledesma-Amaro, Jian Chen, Long Liu

Journal Article